Sequencing Services
Sequencing Services
**Ion S5 XL (Ion Torrent) amplicons
All Ion S5 XL projects are being migrated to the Illumina MiSeq and Illumina NovaSeq Sequencing Platforms
All economy sequencing projects previously completed using the Ion S5 will now be completed using the Illumina MiSeq and NovaSeq at no additional cost.
- The same 515F-806R and ITS1-2 primers will continue to be made available for use on all Illumina sequencing platforms
- Contact us to discuss additional Illumina specific primer sets and pricing options
300bp 515F bacterial + archaeal or ITS1-2 fungal diversity Ion S5 assays with nominal 15-20,000 reads/assay for $60/assay (any size project)
>400bp bacterial, archaeal, or ITS fungal diversity assays with nominal 15-20,000 reads/assay starting at $85/assay and continues to follow the Illumina MiSeq pricing scale below
Illumina MiSeq:
2x300bp PE illumina 20,000 sequence diversity assays starting $85/assay 1-50 assays (note: for projects < 10 assays per library, a $100 library fee is added), $80 for 50-100 assays, 75 for 100-150 assays, $70 for 150-300, $65 for > 300 assays.
PacBio SEQUEL:
Long read amplicons (e.g. near full 16s ~1400bp, ITS1-4, 18s ~1700bp or other custom amplicon diversity (700-2500bp) ( usually minimum 10 samples or $150 library fee is added )
Academic and Government Pricing
$90 per sample for 5,000 sequences per assay,
$125 per sample for 10,000 sequences per assay
$200 per sample for 20,000 sequences per assay
$340 per sample for 40,000 sequences per assay
**Custom assays from 700bp - 3000bp or larger of course have a barcoding fee that is typically $15/barcode
Here are examples of Genome and metagenome programs. Note that we do have bulk discounts for large projects.. these examples are for small projects e.g. < 5 samples
*** Genomes or Metagenome shotgun DNA :
- 2x150bp 10-20 million paired sequences per sample = $400 (data only)
-2x300bp 1-2 million sequences = $600 (data only)
-2x250bp 10 million sequences = $650(data only)
-2x250bp 4 million sequences = $450(data only)
NEW: gap closure sequencing long read scaffolding = $800/sample
Data assembly with optimization = $150
-----we have all kinds of bioinformatics services for shotgun platforms just ask ;-)
Here are examples for sequencing RNA samples such as RNAseq and metatranscriptomes.. These are small project prices but we do have bulk discounts for large projects.
***Transcriptome Metatranscriptome shotgun RNAseq:
- 2x150bp 10-20 million paired sequences per sample = $460 (data only)
- 2x300bp 1-2 million sequences = $700 (data only)
- 2x250bp 10 million sequences = $710 (data only)
- 2x250bp 4 million sequences = $510 (data only)
--- Ribosomal depletion if needed for metatranscriptomes or bacterial transcriptomes= $300
DNA Extraction / RNA Extraction Price:
--DNA Extraction starting at $25 / sample (depending on service requested and sample type submitted)
--RNA Extraction starting between $30 - $70 / sample (depending on service requested and sample type submitted)
| Sequencing Platform | Read Length /Assay Length | Pricing |
| Ion S5 XL** | 300bp 515F bacterial + archaeal or ITS1-2 fungal diversity | For nominal 15-20,000 reads/assay: |
| $60/assay (any size project) | ||
| Ion S5 XL (EXT)** | >500bp bacterial, archaeal, | Same Pricing as Illumina MiSeq |
| or ITS fungal diversity assays | (No Additional Library Fee) | |
| 20,000 sequence diversity assays starting at: | ||
| $60 /assay (>150 assays) | ||
| $65/assay (100-150 assays) | ||
| Illumina MiSeq | 2x300 PE | $70/assay (50-100 assays) |
| $75/assay (20-50 assays) | ||
| $80/assay (1-20 assays) | ||
| For projects < 10 samples a $100 library fee is added | ||
| Low Coverage (< 500 Seqs): $50/assay | ||
| ~5,000 Seqs: $90/assay | ||
| PacBio Sequel | Long Read Amplicon Sequencing | ~10,000: $125/assay |
| (16s, 18s, ITS, and Custom) | ~20,000: $200/assay | |
| ~40,000: $340/assay | ||
| Custom Coverage available;
Contact us for pricing |
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| For projects < 10 samples a $150 library fee is added & $20/sample PCR replication fee |
Communal Genomes or Metagenome:
Our new program provides $400 bacterial genomes and metagenomes. This program provides roughly 10 million paired sequences per sample. It is important to note the turnaround for these vary from a few weeks to a few months, therefore this program is only recommended for projects not on a strict deadline.
Microbiome Analyses:
A free comprehensive taxonomic analysis comes with any 16s, ITS, and 18s data (We are also able to provide analyses for several functional genes as well). Included is a full analysis pipeline comprised of denoising, chimera check, low quality sequence cleaning or removal, clustering, otus generation, taxonomic assignment etc. Our pipeline generates about 12 different analysis files evaluating the taxonomic data at each taxonomic level: genus, species, family, class, order, and phylum. Additionally, OTU files are provided with lineage and identity information as well as other statistical values. To aid in navigating all of the analyses and raw data, a readme help file is provided.
For an additional $10/sample (to recover cost only) we perform alpha and beta diversity, rarefaction, unifrac PCoA etc. These analyses are provided raw, but a standard readme help file is provided, making it fairly easy to work through this extensive amount of data.
We also offer advanced analysis and report generation. Similar to a research manuscript, the report will contain methods, biostatistics, figures, tables, legends, results section, and findings overview (if relevant). The cost for advanced analysis and report generation begins at $500 and may increase depending on the size and complexity of the experiment. Please detail fully your experimental design and the variable information for your samples to give us the ability to accurately quote these costs. All report generation and other bioinformatics are billed based upon the time required. We are fast and always make the cost a great value!
THE FOLLOWING OPTIONS are also available for targeted amplicon sequencing on each of our platforms:
archaea
nifH
HIV
MHC
fungi
protozoa
AND MUCH MORE!
Our analysis tools are mature for Illumina, Ion PGM / S5, and PacBio Sequel. For functional genes some setup costs may be necessary for analysis to create a taxonomic database.
