Sequencing Services

MR DNA specializes in any type of amplicon sequencing.

 

From 16s, 18s, ITS, functional genes to any type of custom primer amplicon assay.

We have an extensive inhouse assay (primer) collection for 16s sequencing, 18s sequencing,  ITS sequencing, functional genes such as nirS, nifH, dsr,  pufM, nosZ, HMC,  and many more.  if you have a custom assay we inexpensively set it up for you.

 

Amplicon Sequencing Prices.

 

MR DNA has all the major sequencing platforms and we have a large selection of amplicon sequencing programs ..  Everything can be customized to the needs of the customer, only limited by the capabilities of the sequencing technologies.

 

Any amplicons (bTEFAP® services) such as 16s, 18s, ITS, functional or custom assays.. if you have an amplicon with custom primers we can help sequence it.

 

Illumina MiSeq and NovaSeq Amplicons

examples for any of our hundreds of inhouse assays

***2x300bp PE illumina 20,000 sequence diversity assays

$80/assay 1 - 20 assays (note: for projects < 10 assays per library, a $100 library fee is added),

$75 for 20 - 50 assays,

$70 for 50 - 100 assays,

$65 for 100 - 150,

$60 for > 150 assays.

additional discounts for very large projects also

 

PACBIO SEQUEL:

NEW:  Long read full 16s ~1400bp amplicon diversity average 5k reads per sample (minimum 20 samples) for $90/sample

 

LONG read ITS1-4 sequencing for fungi

 

long read 18s  1700bp spanning many variable regions  (several options available)

 

long read custom assays from 600bp up to 8000bp  (custom assay setup costs

range from $15-$20/barcode)

 

 

Barcoded amplicon sequencing has become a popular sequencing solution for scientists interested in a specific genomic region.  Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run. Due to its popularization, barcoded amplicon sequencing can now be completed through a variety of different processes utilizing several different technologies. MR DNA has been at the forefront of this technology and through calculated fine tuning, we are proud to offer an inexpensive sequencing method known as bTEFAP®. Originally designed to employ 16s eubacterial primers to be sequenced via pyrosequencing technology, we have expanded its low cost sequencing capabilities to include Archaea, Fungi, and functional gene primers compatible to be sequenced across all of our sequencing platforms. The increased flexibility and versatility of bTEFAP® gives us the opportunity to provide our customers with the lowest sequencing cost possible.

In The News

A New Species

The one bacteria most everyone is familiar with, and maybe without even knowing they are...Clostridium difficile. This diarrhea causing bacteria, C. difficile, may be splitting into two. Researchers from the Wellcome Sanger Institute have just completed a large scale research project evaluating this pesky little bug with specific concern to its presence within hospital environments. This study involved sequencing the DNA of 906 strains of C. difficile collected across 33 countries, and as a result of this collective effort, scientists suggest that a new bacterial species is emerging, which is currently known as C. difficile clade A.

 

This potential new species was found in 70% of samples collected in hospital patients and of course one of the key differences in C. difficile clade A is its resistance to hospital disinfectants. It is clear that DNA sequencing is elucidating the seemingly infinite number of micro-environments that exist in our world today. It is no secret that the word "microbiome" is all the buzz in our circles recently and for good reason. As technology increases and cost decreases, we are discovering just how much our world is influenced by the bacteria that surrounds us everyday. Microbiology, Ecology, Immunology, Agricultural Science, Citizen Science etc. the list could go on and on with regards to the research areas that are growing as a result of DNA Sequencing. Speciation events such as the one discoverd in Clostridium difficile is just one example as to how the power of DNA sequencing is making large scal research studies possible.

 

Reference:

Sanger. Diarrhoea-causing bacteria adapted to spread in hospitals. Retrieved from https://www.sanger.ac.uk/news/view/diarrhoea-causing-bacteria-adapted-spread-hospitals

 

 

 

Microbiome Sequencing Services | Molecular Research MR DNA

At Molecular Research MR DNA, we specialize in microbiome sequencing to uncover the diversity, taxonomy, and functional potential of microbial communities in various environments. Our services cater to a wide range of applications, from human health and agriculture to environmental science and industrial microbiology. Using advanced sequencing technologies, we target bacterial, archaeal, fungal, and eukaryotic microbiomes with markers such as 16S rRNA, ITS, and COI, providing accurate and detailed insights into microbial ecosystems.

For bacterial and archaeal communities, 16S rRNA sequencing is a powerful tool for taxonomic profiling and diversity analysis. Fungal microbiomes are analyzed using ITS sequencing, enabling detailed studies of mycobiomes in soil, plants, and host organisms. COI sequencing expands our capabilities to explore eukaryotic microbes, providing insights into biodiversity, ecosystem roles, and microbial interactions. Additionally, we offer functional gene marker analysis to investigate metabolic pathways, nitrogen cycling, carbon utilization, and antimicrobial resistance within microbiomes.

Our microbiome services are complemented by robust bioinformatics support, including taxonomic classification, diversity metrics, functional annotation, and pathway analysis. From gut microbiome studies exploring human health and disease to environmental research on soil, water, and air microbiomes, Molecular Research MR DNA delivers comprehensive solutions tailored to your scientific goals. With a commitment to high-quality data and reliable results, we empower researchers to make significant discoveries in microbiome science.

Whether you are conducting longitudinal microbiome studies, evaluating the impact of environmental changes, or investigating microbial contributions to host health, Molecular Research MR DNA is your trusted partner. Our expertise in sequencing, bioinformatics, and microbiome research ensures that your projects are supported with precision and innovation. Let us help you uncover the intricate world of microbiomes and advance your understanding of microbial ecosystems.

MRDNA Bacteria Sequencing Contact Number