Genome Sequencing
Sequencing Services
From Bacterial and Fungal genome sequencing to human Whole Genome Sequencing (WGS), MR DNA has you covered!!
MRDNA has a wide array of NGS platforms to accomplish your sequencing goals, including the Illumina NovaSeq 6000 and the PacBio Sequel IIe.
So no matter the project size, large or small, MR DNA is dedicated to offering our customers the flexibility to achieve the desired library size and desired sequencing coverage at the lowest cost possible.
Pricing. for best price just contact us info@mrdnalab.com. quick and direct responses!
Only have a few samples to sequence? No problem!!
- single sample Bacterial Genomes can be sequenced for as low as $250 for larger projects
D iscounts are available for projects with > 5 genomes
- Mid-range size projects (50 - 100 small genomes) can be sequenced for as low as $320 depending on the options needed.
You have a big study with a large number of samples? Great!!
- Bulk pricing for small genomes (> 100 genomes) often as low as $250 per sample
- Contact us for more information on bulk discounts
Small Eukaryote genomes start at $500 in our genome service, see below for more information and example programs.
Comprehensive Genome Sequencing Services: Unraveling the Blueprint of Life Advanced Technologies for Precise, High-Throughput Sequencing At MR DNA , we specialize in genome sequencing solutions, utilizing the latest in high-throughput sequencing technologies to deliver accurate and detailed insights into the genetic code. Our expertise spans multiple genomic sequencing techniques, allowing researchers and clinicians to explore genetic variations, identify mutations, and unlock new possibilities in fields from healthcare to biotechnology. Our Genome Sequencing Techniques Our genome sequencing services cover a wide range of cutting-edge techniques, ensuring comprehensive data collection and high-resolution analysis. We employ various methods based on project needs, offering precision, efficiency, and scalability: 1. Whole Genome Sequencing (WGS) Whole Genome Sequencing provides a complete map of the genetic material in an organism. This technique covers both coding and non-coding regions of the genome, delivering an in-depth view of genetic variation, structural variants, and mutation profiles. Our WGS services utilize advanced next-generation sequencing (NGS) platforms for fast, accurate, and cost-effective results. Keywords: whole genome sequencing, NGS, genetic variation, structural variants, high-throughput, mutation detection 2. Targeted Sequencing Our targeted sequencing services focus on specific genes or genomic regions, enabling detailed examination of areas associated with diseases, traits, or specific functions. By concentrating on targeted gene panels or customized regions, this method offers efficient, high-depth coverage, ideal for studies in oncology, rare diseases, and pharmacogenomics. Keywords: targeted sequencing, gene panels, custom sequencing, high-depth coverage, oncology, pharmacogenomics, rare diseases 3. Exome Sequencing Whole Exome Sequencing (WES) concentrates on protein-coding regions (exons) to uncover protein-altering mutations linked to diseases. WES is a cost-effective way to focus on the regions that most directly impact phenotypes and disease expression, delivering high-quality data for disease research and clinical applications. Keywords: whole exome sequencing, WES, protein-coding regions, disease expression, cost-effective sequencing, clinical genomics 4. Metagenomic Sequencing Our metagenomic sequencing services identify and classify diverse microorganisms within complex samples, like environmental or human microbiome samples. This powerful technique enables comprehensive analysis of microbial communities without culturing, providing insights into biodiversity, microbial functions, and interactions. Keywords: metagenomic sequencing, microbiome analysis, biodiversity, microbial classification, shotgun sequencing, environmental genomics 5. RNA Sequencing (RNA-Seq) Our RNA sequencing (RNA-Seq) services deliver valuable insights into gene expression and transcriptomics. By examining the transcriptome, researchers can better understand cellular processes, gene regulation, and discover new RNA biomarkers. RNA-Seq is critical for studies in cancer research, developmental biology, and more. Keywords: RNA sequencing, RNA-Seq, transcriptomics, gene expression, transcriptome, biomarker discovery, gene regulation 6. Single-Cell Sequencing Single-cell sequencing allows the analysis of individual cells, revealing cell-specific variations and enabling precision insights into complex tissues and heterogeneous populations. Ideal for studies in oncology, immunology, and neuroscience, single-cell sequencing unveils cellular heterogeneity and rare cell types within a sample. Keywords: single-cell sequencing, cellular heterogeneity, single-cell RNA-Seq, precision genomics, rare cell types, oncology, immunology Applications of Genome Sequencing Genome sequencing has transformative applications across research and clinical fields, empowering advances in: Oncology: Identify cancer mutations, understand tumor heterogeneity, and track clonal evolution with targeted and whole-genome sequencing. Genetic Disease Research: Detect rare genetic mutations and structural variations associated with inherited diseases and syndromes. Microbiome Analysis: Investigate complex microbial ecosystems with metagenomic sequencing for insights into health, agriculture, and environmental science. Pharmacogenomics: Discover genetic variants affecting drug response, enabling personalized medicine and optimized treatment plans. Agrigenomics: Analyze genomes of plants and animals to improve breeding, productivity, and resilience to environmental challenges. Keywords: oncology, cancer genomics, genetic mutations, microbiome analysis, pharmacogenomics, agrigenomics, personalized medicine Why Choose Molecular Rds? With extensive experience in genome sequencing technologies, MR DNA provides end-to-end sequencing and bioinformatics support. Our expertise in NGS, WGS, WES, RNA-Seq, and single-cell sequencing allows us to offer tailored solutions to meet your research or clinical objectives. Advanced Platforms: We utilize the latest sequencing technology for high-resolution and high-throughput data. Comprehensive Bioinformatics: Expert analysis, variant calling, pathway analysis, and data visualization ensure meaningful results. Flexible and Scalable Solutions: Customizable workflows for projects of all sizes, from pilot studies to large-scale sequencing efforts. Secure Data Management: Adhering to strict data security protocols to protect your genomic information. Keywords: genome sequencing, next-generation sequencing, bioinformatics, variant calling, data analysis, data security, customized workflows Ready to Sequence with Us? Unlock the potential of your genomic data. Contact MR DNA today to learn how our genome sequencing services can advance your research or clinical discoveries.
* Price will vary based on sequencing coverage required
Shotgun sequencing and de novo assembly of genomes at MR DNA.
MR DNA has many programs for sequencing DNA and RNA (RNAseq, metagenomes, metatranscriptomes, eukaryote genomes, prokaryote transcriptomes, bacterial genomes, virus, phage, plasmid, fosmid etc. (see our transcriptome sequencing page for more on RNA sequencing)
BULK AND LARGE PROJECT DISCOUNTS AVAILABLE: below are program examples for small projects and minimum scale sequencing, with only a few samples, but definitely with larger numbers of samples or samples requiring much more data we definitely can help and provide volume and bulk discounting for some of the lowest price fastest turnaround commercial, academic and government rates available anywhere!
Here are just a few examples of shotgun sequencing programs but do contact us for customized projects especially if you have more than a few samples or need larger amounts of data .. we are here to work hard and help!
BIOINFORMATICS FOR GENOMES
DNA Extraction / RNA Extraction Price:
Our aim is to deliver research teams with their desired amount of data at the lowest cost possible. We understand that project sizes can vary; from probative studies containing a small number genomic samples to large-scale population studies containing > 100 genomes, and we are here to work with you! With high-output sequencers such as the Illumina NovaSeq and the PacBio Sequel at your disposal, you can rest assured that we will do our best to deliver the maximum output at the lowest cost to you. The prices and data capabilities below represent the typical requests we receive from customers. As members of the scientific community, we understand that not every project is the same. So whether you need 6Tb of data or maybe a more affordable price to complete your large study, don't hesitate to give us a call to see how we can meet your specific needs.
Benefits of Whole Genome Sequencing
- Ability to provide greater insight into personalized medicine and diagnosis
- Aid to uncover the genetic causality of rare and/or inherited diseases
- Provides researchers with a more balanced coverage in comparison to Whole Exome Sequencing
Whole genome sequencing allows scientists to elucidate the entirety of the genome of a specific organism. As the cost of DNA sequencing continues to decrease, due in large part to Next Generation Sequencing, the more affordable and cost effective whole genome sequencing becomes for researchers everywhere. Our experience in whole genome sequencing, paired with a comprehensive portfolio of sequencing platforms, allows us to provide you with the proper work flow for any project. From bacterial genomes to the human genome, we are dedicated to providing our customers with the most reliable and affordable sequencing solution possible. Feel free to contact us for more information.
Why Should I Choose Whole Genome Sequencing?
One key benefit to choosing whole genome sequencing (WGS) over targeted sequencing methods is that WGS allows researchers an all-inclusive look at the genome in question. Where previous sequencing technologies and other methods lack, NGS platforms excel. High-throughput sequencing allows researchers to detect small and rare genetic mutations such as INDELs and SNPs. With the increased sensitivity that NGS platforms bring to whole genome sequencing, the field of personal medicine is exploding. Cutting-edge research is being performed in fields such as Oncology and Immunology with the goal in mind of not only treating a certain cancer or auto-immune disease as a whole, but formulating a treatment plan based on the individual with that certain diagnosis.
Now don't limit yourselves to only thinking that WGS is specifically for large genomes . Due to the scalability of certain NGS platforms, the power of whole genome sequencing can be applied to research of bacteria, fungi, and other microbial organisms. The sequencing depth achieved by NGS platforms allows researchers to truly take advantage of whole genome sequencing when it comes to sequencing small genomes, such as bacterium, because several bacterial genomes can be sequenced in parallel saving both time and money.
In The News
In a recent interview conducted by Illumina the Illumina NovaSeq is highlighted as a key instrument in the of future agriculture genomics. According to Pew Research, "By 2100, the world’s population is projected to reach approximately 10.9 billion", and a large question for farmers across the globe is, "How are we going to feed everyone?" By utilizing sequencing instruments such as the HiSeq 2500 and the NovaSeq 6000, researchers are able to undertake research projects involving genomes such as wheat, cotton, corn, sorghum, etc. As high throughput sequencers, such as the NovaSeq 6000, continue to grow in their data generation capabilities, the cost of whole genome sequencing continues to decrease, and as a result, WGS of plant species is now commonplace. From identifying novel SNPs to genotyping fungal and insect pathogens, NGS and whole genome sequencing will continue to revolutionize the agrigenomics industry year after year.
References
Illumina.com. (2019). Agriculture Throughout the World is Benefiting from the Latest NGS Technologies. [online] Available at: https://www.illumina.com/science/customer-stories/icommunity-customer-interviews-case-studies/johnson-agrilife-interview-ag-novaseq.html [Accessed 5 Jul. 2019].
Cilluffo, A., Ruiz, N. G., Cilluffo, A., & Ruiz, N. G. (2019, June 17). World population growth is expected to nearly stop by 2100. Retrieved from https://www.pewresearch.org/fact-tank/2019/06/17/worlds-population-is-projected-to-nearly-stop-growing-by-the-end-of-the-century/
Why Should I Choose Whole Genome Sequencing?
Ten years ago this would have been a valid question, and to some regard still is, but a more fitting question for the age of technology we find ourselves in today is, "Why NOT choose whole genome sequencing?". Ten years ago, according to the NIH, the cost per genome hovered right around $10M, but due to scientific and technological advancements the $1,000 genome is now a reality. What used to be reserved for large corporations and research projects receiving astronomical funding is now available to even the smallest university labs. A major impact upon the cost of whole genome sequencing is the advancement of sequencing technology itself. High-throughput sequencing, also known as next-generation sequencing (NGS), has made whole genome sequencing largely accessible due to its capability for producing millions of reads. The capability of NGS instruments, such as the Illumina HiSeq, to produce millions of reads is what truely unharnesses to the power of whole genome sequencing; deep sequencing coverage.
One key benefit to choosing whole genome sequencing (WGS) over targeted sequencing methods is that WGS allows researchers an all-inclusive look at the genome in question. Where previous sequencing technologies and other methods lack, NGS platforms excel. High-throughput sequencing allows researchers to detect small and rare genetic mutations such as INDELs and SNPs. With the increased sensitivity that NGS platforms bring to whole genome sequencing, the field of personal medicine is exploding. Cutting-edge research is being performed in fields such as Oncology and Immunology with the goal in mind of not only treating a certain cancer or auto-immune disease as a whole, but formulating a treatment plan based on the individual with that certain diagnosis.
Now don't limit yourselves to only thinking that WGS is specifically for large genomes . Due to the scalability of certain NGS platforms, the power of whole genome sequencing can be applied to research of bacteria, fungi, and other microbial organisms. The sequencing depth achieved by NGS platforms allows researchers to truly take advantage of whole genome sequencing when it comes to sequencing small genomes, such as bacterium, because several bacterial genomes can be sequenced in parallel saving both time and money.
Sequencing Applications
| Sequencing Platform | Read Length /Assay Length | Genome Sequencing Pricing |
| Illumina NovaSeq 6000 ** | 2x150bp | 10 Million PE (Paired-End) sequences / sample = $500 (data only) |
| 30 Million PE (Paired-End) sequences / sample = $800 (data only) | ||
| 70 Million PE (Paired-End) sequences / sample = $1200 (data only) | ||
| Illumina HiSeq | 2x250bp | 10 Million PE (Paired-End) sequences / sample = $900 (data only) |
| 4 Million PE (Paired-End) sequences / sample = $600 (data only) | ||
| Illumina MiSeq | 2x300 bp | 1-2 Million PE (Paired-End) sequences / sample = $600 (data only) |
| PacBio Sequel | 10-Hour Movie (Run) Time | * Low Coverage Bacterial Genomes starting at $800 |