Shotgun sequencing and de novo assembly of genomes or transcriptomes at MR DNA.

Reference assembly and long read genomes 

 

MR DNA has many programs for sequencing DNA and RNA (RNAseq, metagenomes, metatranscriptomes,  eukaryote genomes, prokaryote transcriptomes,  bacterial genomes, virus, phage, plasmid, fosmid etc.

 

THIS LIST of programs is for small projects and minimum scale sequencing, with only a few samples, but definitely with larger numbers of samples or samples requiring much more data we definitely can help and provide volume and bulk discounting for some of the lowest price fastest turnaround commercial, academic and government rates available anywhere!

 

here are just a few examples of shotgun sequencing programs but do contact us for customized projects especially if you have more than a few samples or need larger amounts of data .. we are here to work hard and help!

 

NOTE:*** Pricing below is for small projects 1-2 samples  discounts start at 3-5 samples and of course large and bulk discounts available for larger projects ..please email us for accurate pricing

*** Genomes or Metagenome shotgun DNA :  1-5 samples (discounts with larger projects)

- 2x150bp 10-20 million paired sequences per sample = $400 (data only)  Illumina NovaSeq

- 2x300bp 1-2 million sequences = $600  (data only)  Illumina NovaSeq

- 2x250bp 10 million sequences = $650 (data only) Illumina NovaSeq

- 2x250bp  4 million sequences = $450 (data only)  Illumina NovaSeq

long read sequencing up to 8000bp (or more) available PacBio Sequel!   contact us for custom pricing on any project.

 

NEW:  gap closure sequencing long read scaffolding for bacteria = $800/sample.. this adds long read data to Illumina sequencing data and may result in much fewer contigs during hybrid assembly.

 

BIOINFORMATICS FOR GENOMES

Data assembly with optimization = $150  (for bacterial genomes we also provide free genome annotation giving you genbank files, proteome files, transcriptome files and genome spreadsheets)

 

 

***Transcriptome, Metatranscriptome, RNAseq, polyA and shotgun total RNA  :

- 2x150bp 10-20 million paired sequences per sample = $460 (data only)

- 2x300bp 1-2 million sequences = $700  (data only)

- 2x250bp 10 million sequences = $710 (data only)

- 2x250bp 4 million sequences = $510 (data only)

--- Ribosomal depletion (new lower pricing) if needed for metatranscriptomes or bacterial transcriptomes= $300

**LONG READ GENOMES WITH PAC BIO sequel IIe

bacterial genomes start at $600 for low coverage and $800 for moderate coverage  

 

BIOINFORMATICS FOR transcriptome or other RNA based projects

usual costs for comparing two groups with RNAseq is $200

usual costs for annotation of a transcriptome with assembly is $200

we have extensive range of bioinformatics services.  just shoot us an email or give us a call of course!

 

DNA Extraction / RNA Extraction Price:

-- DNA Extraction starting at $40 / sample (depending on service requested and sample type submitted)

-- RNA Extraction starting between $50 - $150 / sample (depending  on service requested and sample type submitted)

 

shotgun and denovo sequencing

It is well known that next generation sequencing applications have led to numerous genomic discoveries that would otherwise have been improbable if not impossible. However, the number of genomes that have yet to be discovered is countless. The identity of these novel genomes is elucidated through De Novo Sequencing. Sequencing and assembly of novel genomes is only made possible with sufficient coverage of the genome of interest along with extended read lengths. While NGS has always offered researches deep sequencing capabilities, as the read length capabilities of next generation sequencers steadily increase, not only does De Novo Sequencing become more reliable, but it is also becomes more affordable. Integrating a seasoned laboratory staff with a bioinformatics team that has 20 years of continuous applied bioinformatics experience allows MR DNA to offer its customers a low cost sequencing solution, not only with regards to reference genomes, but novel genomes as well.

 

At Molecular Research MR DNA, we provide cutting-edge de novo and reference genome sequencing services to support diverse fields of research and industry. De novo sequencing allows for the construction of novel genomes without prior reference, enabling the discovery of unique genomic features in uncharacterized species. Our high-throughput platforms ensure accurate and complete genome assembly, offering unparalleled insights into genome structure, gene content, and evolutionary relationships. From microbial genomes to complex plant and animal genomes, our solutions are tailored to meet the needs of your specific research objectives.

Reference genome sequencing enhances understanding by comparing samples to well-characterized genomes. This approach is ideal for detecting structural variations, single nucleotide polymorphisms (SNPs), and other genomic changes. Our expertise in whole-genome resequencing supports population studies, evolutionary biology, and disease research. With high-depth sequencing and robust bioinformatics, we deliver precise data for comparative genomics, functional analysis, and genome-wide association studies (GWAS). Our services enable researchers to explore genomic diversity, identify conserved regions, and analyze mutations with confidence.

Our bioinformatics pipeline is designed to streamline genome assembly, annotation, and analysis. For de novo genomes, we provide tools for scaffolding, gap closure, and repeat resolution. Reference genome projects benefit from precise read alignment, variant calling, and structural annotation. Whether your focus is on agricultural improvements, biomedical research, or environmental applications, Molecular Research MR DNA is committed to delivering high-quality genome sequencing solutions. With state-of-the-art technology and expert support, we help you unlock the potential of genomic data to drive innovation and discovery.

 

At Molecular Research MR DNA, we leverage both Illumina and PacBio Sequel platforms to provide comprehensive genome sequencing solutions. Illumina sequencing is renowned for its high-throughput capabilities, offering short-read data with exceptional accuracy. This platform is ideal for resequencing, transcriptomics, and detecting small variants, such as single nucleotide polymorphisms (SNPs) and insertions/deletions (indels). PacBio Sequel, on the other hand, utilizes long-read sequencing to resolve complex genomic regions, structural variations, and repeat sequences, making it a powerful tool for de novo genome assembly and epigenetic analysis.

By combining Illumina and PacBio technologies, we deliver hybrid genome assemblies that maximize data accuracy and completeness. Illumina's short-read precision complements PacBio's long-read capabilities, enabling robust assembly of even the most challenging genomes. This dual-platform approach is particularly advantageous for studying complex plant and animal genomes, microbial communities, and structural genomic rearrangements. Whether your project involves comparative genomics, metagenomics, or population studies, our tailored sequencing strategies ensure that you achieve reliable and high-quality results.

Our comprehensive bioinformatics pipeline supports the analysis of Illumina and PacBio data, from raw read processing to advanced genome annotation. For Illumina data, we provide high-resolution variant detection, gene expression profiling, and metagenomic taxonomic classification. With PacBio data, we offer tools for haplotype phasing, repeat annotation, and base modification detection. At Molecular Research MR DNA, we are committed to delivering state-of-the-art sequencing solutions that meet the needs of diverse research and industrial applications. Let us help you harness the full potential of Illumina and PacBio Sequel technologies for your genomic research.